Composite

Part:BBa_K1593999:Design

Designed by: Yuting Liu   Group: iGEM15_USTC   (2015-09-03)


R0010-cheZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 581
    Illegal NgoMIV site found at 716
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the lac promotor to control the expression of cheZ,in this way,we can control the quantitly of expressed cheZ .


Source

The promotor comes from the part,BBa_R0010.The cheZ comes from the genomic sequence of PAO1.

References